| 1. | We have identified six conserved domains by multiple sequence alignments of the thymidine kinase proteins of the alphaherpesviruses 通过对疱疹病毒tk氨基酸进行多序列比较,我们获得了在prvtk氨基酸序列上的六个保守区间。 |
| 2. | The secondary structure of this protein speculated had a typically conserved domain of cytochrome p450 cysteine heme - iron ligand signature ( fnagprlcig ) and a hydrophobic domain at n - terminal of amino acid ( 6 ) c钾86mf同源基因片段在13个物种中的序列分析表明:它们的核昔酸差异为1 |
| 3. | In part two , amino acid sequence of haorf2 was analysed , and conserved domains of wasp protein were found in haorf2 , which suggests haorf2 functions similarly to wasp family proteins 第二节对ha2基因的结构与功能的研究进行了报道。 ha2蛋白感染hzami细胞后24h可以检测到蛋白表达,表明ha2是晚期表达基因。 |
| 4. | Comparison of conserved domains ( core - 2 / i - branching and wsc domain ) of oxt with mammalian homologues showed that both of domains are conserved throughout the invertebrate and vertebrate 将ox亡蛋白的保守结构域和哺乳动物的同源基因的保守结构域进行比较,发现这些保守区域在所比较的脊椎动物和无脊椎动物中都十分保守。 |
| 5. | The residues capable of forming amphipathic a - helice structure in the first conserved domain are identical to those of ap2 , while the structure in the second conserved domain lacks 9 amino acids as compared to that of ap2 螺旋的氨基酸残基与apz的完全相同,而另一个区域中形成。螺旋g :氦基酸殁基与apz相比少9个氨基酸。 |
| 6. | The high homology genes are not found in all genes known . but the putative conserved domains of peptidase have been detected . in the domains the homology comparing with callus gallus cathepsin b cdna is 90 % Ncbi检索未见同源性高的基因序列,但发现含有典型的肽酶结构域,编码蛋白与组织蛋白酶b结构域部分一致性高达90 ,其具体功能有待于进一步验证。 |
| 7. | According to the conserved domain of the pal gene family , we achieved to the segment of pal gene about 950bp in rhodiola crenulata . some proposals were put forward for further development of the important enzymes in the metabolic pathway of salidroside 我们还根据苯丙氨酸解氨酶( pal )基因家族的保守序列,以大花红景天dna为模板,通过pcr扩增得到其约950bp长的pal基因的克隆,测序并比较了它与其基因家族的同源性。 |
| 8. | Comparing with database in genbank , the results showed ej175 had 56 % homology to mrna for a known pollen allergen - like protein in arabidopsis thaliana . ej175 was proved to encode gene of pathogenesis - related protein bet v i family by conserve domain analysis 对该基因可读框编码的蛋白进行结构功能域分析,证实了该基因4 - 147位氨基酸存在病程关联蛋白betvi家族( pathogenesis - relatedproteinbetvifamily )的的保守结构域bet - v - i 。 |
| 9. | The protein encoded by orf1 show strong homology with cation efflux family protein from geobacter sulfurreducens pca [ identities = 65 / 272 ( 23 % ) ] , and contains mmt1 - like putative conserved domains kog1485 , mmt1 contains conserved domains kog1485 is mitochondrial fe2 + transporter ( cation diffusion facilitator superfamily ) . so it can be considered that the protein encoded by o rf1 is fe2 + transporter involved in magnetosome biosynthesis in magnetospirillum gryphiswaldense msr - 1 经同源比较和保守性结构域分析,推测orf1编码的蛋白具有fe ~ ( 2 + )转运功能,在磁小体合成过程中将细胞质中fe ~ ( 2 + )转运到磁小体囊泡中,合成磁小体的同时去除fe ~ ( 2 + )对细胞的毒害作用。 |
| 10. | The dna sequence had a complete orf ( open reading frame ) , which coded a protein of 479 amino acid residues . the protein sequence of enolase which contained the conserved domain , was homologue to enolase of other organisms . it showed 83 % ideaity and 89 % similarity compared to the enolase in chlamydomonas reinhardtii 并将其中的一个gus基因用目的片段烯醇酶基因替换,构建了可以在植物中高效表达的载体pcambia2301g一enolase ,成功地将其转入根癌农杆菌eha105中,为下一步进行转基因植物的研究作准备。 |